Core functions of an autoclave in the Lab

An autoclave utilizes saturated steam at temperatures between 121C and 134C to destroy the cell membranes of microorganisms. You should know that wet sterilization is the core function, as it allows steam to penetrate deeply into hollow instruments.

Many modern autoclave models integrate vacuum drying. This shortens the cycle time from 60 minutes down to just 20 minutes, saving you valuable time. The Zonkia LX-B35L Digital Autoclave features an LED screen for real-time monitoring. The machine automatically shuts off after maintaining 121C for 25 minutes, making it ideal for small labs.

Common Operating Modes

Class B autoclaves, such as the STURDY SA232, support three primary modes: solid instruments, wrapped fabric, and waste. Once you select the mode, the vacuum pump removes air, allowing steam to distribute evenly.

The SH Scientific SH-AC-60M with a 60-liter capacity is equipped with dual safety valves. It reaches a pressure of 2.1bar, suitable for large labs with high sterilization demands. Furthermore, audible alarms and electronic door locks prevent premature opening. These functions make the autoclave a highly reliable piece of equipment.

Common specimens requiring autoclave sterilization

Test tubes, pipettes, and Petri dishes are the primary specimens that require an autoclave. You must sterilize them before an experiment to prevent microorganisms from skewing culture results. Agar media and metal tools like forceps are also mandatory for sterilization.

Biological waste, such as gauze, requires an autoclave to be rendered harmless before disposal. In microbiology labs, an autoclave can quickly process 20-30 test tubes. The results remain pure, ensuring accurate DNA research.

Safety notes for operating an autoclave

Always check the rubber gaskets and distilled water levels before running a cycle. By avoiding pressure imbalances, you can extend the machine's lifespan to 5-7 years. Do not pack instruments too tightly; leave enough space for steam circulation.

With the Zonkia LX-B35L, using stainless steel baskets optimizes the 35-liter chamber. Maintenance should be performed every 6 months, specifically checking valves and sensors. Following the correct procedure ensures your autoclave operates smoothly and safely.